Enhanced expression of the Epstein-Barr virus latent membrane protein by a recombinant vaccinia virus.

نویسندگان

  • J P Stewart
  • I N Hampson
  • H W Heinrich
  • M Mackett
  • J R Arrand
چکیده

The complete coding sequence of the Epstein-Barr virus strain B95-8 latent membrane protein (LMP) was cloned using a Raji cell cDNA library and genomic B95-8 DNA. The clone was characterized by sequencing and then used to make a recombinant vaccinia virus. This virus (VLMP) was shown to express a relatively high level of LMP in an authentic fashion. Antisera raised in rabbits against VLMP were shown to react with B95-8 LMP as well as cross-reacting with a 50K cellular protein.

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عنوان ژورنال:
  • The Journal of general virology

دوره 70 ( Pt 5)  شماره 

صفحات  -

تاریخ انتشار 1989